GENESTA™ 1kb DNA Ladder

The 1 Kb DNA Ladder is designed and provided as molecular weight standards for agarose gel electrophoresis. The ladder consists of 13 fragments ranging in length from 250 to 12000 bp. The 1500 bp and 4000 bp bands have increased intensity to serve as reference bands. The approximate mass for each band is 50 ng DNA, and 150 ng for reference band, in the 5 ul sample. The ladder is dissolved in 1x loading dye and ready-to-use. 5x Loading Dye is supplied as a free gift. STORAGE : Store at -20 . The product is stable for 1 year stored at -20 . It can be stored at least 3 months at 4 . Do not heat before loading. PROTOCOL : 1. Load 5 ul ladder for a gel lane, and adjust loading volumeaccording to the width of the agarose gel. 2. Use 0.7 - 1.2 % agarose, and 4 - 10 v/ cm, in 1xTAE electrophoresis buffer. 3. Visualize DNA by EB ( ethidium bromide) staining, or SYBR Green 4. Size : 500 µ  5x Loading Dye : - 10 mM Tris - HCl, - 5 mM EDTA, pH7.6, - 0.03 % Bromophenol blue, - 0.03 % Xylene cyanol, - 30 % Glycerol.
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